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Possible Answers: TRIS.
Random information on the term “TRIS”:
Tris, or tris(hydroxymethyl)aminomethane, or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes.
Tris has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.5 and 9.0.
Tris is prepared industrially by the exhaustive condensation of nitromethane with formaldehyde under basic conditions (i.e. repeated nitroaldol reactions) to produce the intermediate (HOCH2)3CNO2, which is subsequently hydrogenated to give the final product.
Tris is extensively used as a biological buffer or a component of buffer solutions. The useful buffer range for tris (7-9) coincides with the physiological pH typical of most living organisms. This makes tris one of the most common buffers in the biology/biochemistry laboratory.